Fig. 7

Inhibition of TOP2A affects the development potential and quality of pre-implantation embryos. A Flow chart depicting the protocol used for obtaining embryos. B Immunofluorescence was used to confirm the efficiency of the TOP2A inhibitor PluriSIn2. Total magnification: 400x. Scale bar: 20 μm C TOP2A inhibitor restricted the development of four-cell embryos and blastocysts. Scale bar: 100 μm. D Staining of nucleus/DNA (Hoechst), epiblast (NANOG), primitive endoderm (GATA4), and total ICM (NANOG and GATA4 merged). Scale bar: 20 μm E qRT-PCR was used to analyse the expression of the inducer of ZGA and zygotic genes to assess the effect of PluriSIn2. Total RNA was isolated from four‐cell embryos. F The effect of PluriSIn2 on mitochondrial superoxide levels in mouse four-cell embryos. Scale bar: 20 μm. G JC-1 staining of mouse four-cell embryos to determine the effect of PluriSIn2 on mitochondrial potential. Scale bar: 20 μm. *P < 0.05, ***P < 0.001. ns no statistical difference