Table 1 The primer sequences used in qRT-PCR
Gene | Forward primer (5’-3’) | Reversed primer (5’-3’) |
|---|---|---|
KLF3-AS1 | AATGAGTGCGTGGAGGAAAT | CCTGGGCAACAGAGTGAGAC |
MCP-1 | GCAGGTCCCTGTCATGCTTC | ACAGCTTCTTTGGGACACCT |
TNFα | GACGTGGAACTGGCAGAAGAG | TTGGTGGTTTGTGAGTGTGAG |
IL-6 | GGTCCAGTTGCCTTCTCCC | GCAACAAGGAACACCACGG |
Sirt1 | GCTGACGACTTCGACGACG | TCGGTCAACAGGAGGTTGTCT |
USP22 | CCATTGATCTGATGTACGGAGG | TCCTTGGCGATTATTTCCATGTC |
miR-185-5p | GCGCGATTGGAGAGAAAGGCAGT | ATCCAGTGCAGGGTCCGAGG |
miR-138-5p | CCCAGGGTCTGGTGCGGAGA | CAGGGGCTGAGCGGTGAGGG |
miR-206 | GGAATGTAAGGAAGTGTGTG | GAACATGTCTGCGTATCTC |
miR-23c | CCAGAAGGACGTAGAAG | CTTCACTGTGATGGGCTC |
miR-223 | GGCAGCACCCCATAAACTGTT | CAGTGCGTGTCGTGTCGTGGAG |
U6 | GCTTCGGCAGCACATATACTAAAAT | CGCTTCACGAATTTGCGTGTCAT |
GAPDH | AGGTCGGTGTGAACGGATTTG | TGTAGACCATGTAGTTGAGGTCA |