Fig. 3
Immunofluorescence images of AAV-GlyRα3, and ATF-3 expression in the DRG. A Expression of green fluorescent protein (GFP) in L5 DRGs was detected by immunofluorescence six to eight weeks after intrathecal AAV-GlyRα3 (2.5 × 1012 vg) injection. Double immunofluorescence staining showing GlyRα3 and NeuN co-localization in the L5 DRG. Positive for GlyRα3 are shown in green, positive for NeuN are shown in blue, merge images of GlyRα3 and neuron are indicated in peacock blue. A injury assessment by ATF-3 expression of DRG tissue sections from CFA (100 μl) subcutaneous hind paw injection. Group intrathecal NaCl plus CFA injection (GNF), group intrathecal AAV (2.5 × 1012 vg) plus CFA injection (GVF), and group intrathecal AAV-GlyRα3 (2.5 × 1012 vg) plus CFA injection (Gα3F) are compared. ATF-3 expression in the DRG was measured by B immunofluorescence and C western blotting. The data represent the means ± SE (n = 6–9 per group). One-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001. ATF-3 indicates activating transcription factor; scale bars: 250 μm