Fig. 1

Change in DC function and mitochondrial PINK1 expression of DCs at different time after CLP. a, b Representative flow cytometric analysis of MHC-II, CD80, and CD86 expression and statistical analysis of relative MHC-II, CD80, and CD86 expression on DCs in each group (n = 4). c IL-12 mRNA expression was analyzed by PCR and statistical analysis of relative mRNA expression of IL-12 on DCs in each group (n = 3). d TNF-α mRNA expression was analyzed by PCR and statistical analysis of relative mRNA expression of TNF-α on DCs in each group (n = 3). e CD4⁺T cells were activated with ConA (5 μg/mL) for 18 h, and then were co-cultured with DCs at a ratio of 1:100. CCK-8 was used to test T cell proliferation. Representative expression of T cell proliferation in each group (n = 5). f, g Representative images of BrdU staining (red) and DAPI (blue) on DCs in sham and CLP group and statistical analysis of positive cells (n = 3). h, i Representative western blots of PINK1 and COXIV in each group and statistical analysis of relative PINK1 expression (n = 5). j Representative fluorescence images of PINK1 expression on DCs in sham and CLP group, including PINK1 protein (red) and nucleus (blue). k Representative fluorescence images of the co-localization of PINK1 and mitochondria on DCs in sham and CLP group, including PINK1 protein (red), mitochondria (green) and nucleus (blue). Results of experiments were shown as the mean ± SD. Statistical significance was assessed using one-way ANOVA analysis with Dunnett’s multiple comparisons test. P values are reported as follows: * < 0.05; ** < 0.01