Fig. 2From: PINK1 protects against dendritic cell dysfunction during sepsis through the regulation of mitochondrial quality controlChange in DC function and mitochondrial PINK1 expression of DCs at different time following LPS treatment. a, b Representative flow cytometric analysis of MHC-II, CD80, and CD86 expression and statistical analysis of relative MHC-II, CD80, and CD86 expression on DCs in each group (n = 4). c TNF-α mRNA expression was analyzed by PCR and statistical analysis of relative mRNA expression of TNF-α on DCs in each group (n = 3). d IL-12 mRNA expression was analyzed by PCR and statistical analysis of relative mRNA expression of IL-12 on DCs in each group (n = 3). e CD4+T cells were activated with ConA (5 μg/mL) for 18 h, and then were co-cultured with DCs at a ratio of 1:100. CCK-8 was used to test T cell proliferation. Representative expression of T cell proliferation in each group (n = 5). f, g Representative western blots of PINK1 and COXIV in each group and statistical analysis of relative PINK1 expression (n = 5). h Representative fluorescence images of PINK1 expression on DCs in Control and LPS group, including PINK1 protein (red) and nucleus (blue). i Representative fluorescence images of the co-localization of PINK1 and mitochondria on DCs in Control and LPS group, including PINK1 protein (red), mitochondria (green) and nucleus (blue). Results of experiments were shown as the mean ± SD. Statistical significance was assessed using one-way ANOVA analysis with Dunnett’s multiple comparisons test. P values are reported as follows: * < 0.05 and ** < 0.01Back to article page