Fig. 3From: PINK1 protects against dendritic cell dysfunction during sepsis through the regulation of mitochondrial quality controlKnockout of PINK1 prevented DC function following LPS treatment. Immature DCs from WT mice and pink1 Knockout mice were cultured by PBS or LPS (1000 ng/mL) for 4 h. a, b Representative western blots of PINK1 and COXIV in each group and statistical analysis of relative PINK1 expression (n = 3). c, d Representative flow cytometric analysis of MHC-II, CD80, and CD86 expression and statistical analysis of relative MHC-II, CD80, and CD86 expression on DCs in each group (n = 4). e TNF-α mRNA expression was analyzed by PCR and statistical analysis of relative mRNA expression of TNF-α on DCs in each group (n = 3). f IL-12 mRNA expression was analyzed by PCR and statistical analysis of relative mRNA expression of IL-12 on DCs in each group (n = 3). g CD4+ T cells were activated with ConA (5 μg/mL) for 18 h, and then were co-cultured with DCs at a ratio of 1:100. CCK-8 was used to test T cell proliferation (n = 5). Results of experiments were shown as the mean ± SD. Statistical significance was assessed using Student’s t test (b) and two-way ANOVA analysis with Sidak’s multiple comparisons test (d–g). P values are reported as follows: # < 0.05 and **, ## < 0.01Back to article page