Fig. 7From: PINK1 protects against dendritic cell dysfunction during sepsis through the regulation of mitochondrial quality controlKnockout of PINK1 prevented DC function and increased apoptosis of DCs after CLP. Splenic DCs were collected from WT mice and PINK1 knockout mice at 24 h after CLP. a, b Representative flow cytometric analysis of MHC-II, CD80, and CD86 expression and statistical analysis of relative MHC-II, CD80, and CD86 expression on DCs in each group (n = 4). c TNF-α mRNA expression was analyzed by PCR and statistical analysis of relative mRNA expression of TNF-α on DCs in each group (n = 3). d IL-12 mRNA expression was analyzed by PCR and statistical analysis of relative mRNA expression of IL-12 on DCs in each group (n = 3). e CCK-8 was used to test T cell proliferation (n = 5). f, g Representative images of BrdU staining (red) and DAPI (blue) on DCs in each group and statistical analysis of positive cells (n = 3). h, i Apoptosis of DCs was tested by PE-Annexin V and 7-AAD. Representative flow cytometric analysis of apoptosis and statistical analysis of DCs apoptosis in each group (n = 3). j, k Representative western blots of cleaved caspase-3 and β-actin in each group and statistical analysis of relative cleaved caspase-3 expression (n = 3). l Survival rate of the mice from each group over 7 days. Results of experiments were shown as the mean ± SD. Statistical significance was assessed using two-way ANOVA analysis with Sidak’s multiple comparisons test. P values are reported as follows: *, # < 0.05 and **, ## < 0.01Back to article page