Figure 4
Expression of representative genes up-regulated in a statistically significant fashion in the airway epithelium of 2nd brushed area compared with the 1st brushed area in individuals whose right lung was brushed 1st (â—‡) and those whose left lung was brushed 1st (â—†). A: Transcription factors c-fos, jun B, and 3 early growth response genes (EGR1, EGR2, and EGR3). B: Two signal transduction genes, dual specificity phosphatases 2 and 5 (DUSP2 and DUSP 5); 1 gene involved in apoptosis, immediate early response 3 (IER3); and 2 examples of housekeeping genes, glyceraldehyde phosphate dehydrogenase (GAPDH) and TATA-box binding factor IID (TFIID), that did not show significant changes in expression levels between the 1st and 2nd brush in most individuals. The ordinate represents the ratio of the expression level in the 2nd brushed area compared with the 1st brushed area.