SV40-Immortalized Human Fibroblasts as a Source of SV40 Infectious Virions

Table 1 Viral titers showed by SV40 VA45-54-2 strain released by immortalized human fibroblast (45-54-2_MRC5) and infected CV-1 cells (45-54-2_CV-1)

^aViral titers were determined in CV-1 permissive cell monolayers (10⁵ cells/monolayer) by the endpoint dilution technique. Cells were observed for lysis to occur from day 4 p.i. to day 24 p.i. Matching results were obtained infecting normal human fibroblasts with the same viral stocks employed in CV-1 cells.
^bCrude indicates the medium of infected cells without any treatment.
^cFiltered indicates the medium of infected cells processed though 45-nm filters.

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