Figure 2

Phenotypic analysis of DCs generated from B-CLL patients and normal donors. A: Immature DCs from B-CLL patients and normal age-matched controls were stimulated with CyC (see Materials and Methods) for 48 h. Subsequently, cells were stained for the indicated surface antigens and analyzed by FACS. Mean fluorescence intensities (MFI) are depicted on the y-axis. B: Mature B-CLL-DCs and normal-DCs (48 h after stimulation with CyC) were analyzed for the expression levels of certain surface molecules. The result shown is representative of more than 4 independent experiments. C: Depicted are the delta MFI of CD54 in normal-DCs (n = 10) and B-CLL-DCs, either all cases (n = 8) on the left or cases with mutated IgV genes (CLL-mutated, n = 6) on the right. The delta MFI represents the difference in mean fluorescence intensity between DCs matured in the presence of CyC and DCs cultured in medium only.