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Figure 3 | Molecular Medicine

Figure 3

From: Glucocorticoid Receptor Mutants Demonstrate Increased Motility Inside the Nucleus of Living Cells: Time of Fluorescence Recovery After Photobleaching (FRAP) Is an Integrated Measure of Receptor Function

Figure 3

(A) Recovery of GFP-GRα fluorescence intensity in the nucleus after photobleaching and calculation of t1/2 COS7 cells were transfected with pRShGRα, treated with 10−6 M dexamethasone, and photobleached in the nucleus. Fluorescence intensity was traced time-sequentially, and the recovery t1/2 was estimated from the recovery curve produced. (B) Representative photobleaching.

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Molecular Medicine

ISSN: 1528-3658