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Figure 1 | Molecular Medicine

Figure 1

From: Selective Inhibition of the Human tie-1 Promoter with Triplex-Forming Oligonucleotides Targeted to Ets Binding Sites

Figure 1

Diagram showing the relative positions of the tandem ets transcription factor duplex target sequences (E-1 and E-2), SmaI and AccI restriction enzyme sites, and reported transcription start site (arrow) of the human tie-1 promoter (37). The sequences of the oligonucleotides encoding the E-1 and E-2 target sites that were annealed and used for electrophoretic mobility shift assays are indicated. The sequences of the antiparallel (relative to the purine strand of the target DNA sequence) triplex-forming oligonucleotides, T1-ap and T2-ap, targeted against E-1 and E-2, respectively, parallel control oligonucleotides (T1-p and T2-p), and parallel pyrimidine-rich (T1-(C/T)-p) oligonucleotide are shown.

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Molecular Medicine

ISSN: 1528-3658