Genome-Level Longitudinal Expression of Signaling Pathways and Gene Networks in Pediatric Septic Shock

Table 4A Gene Networks Downregulated Uniquely on Day One

		Panther Classifications³	D.A.V.I.D. Functional Annotation⁴
		• Pathway (P value)	• Category
		• Biological Process (P value)	• Term
Network and Central Node¹	Ingenuity Annotations²	• Molecular Function (P value)	• P value
Network 1 BCL2⁵	• Hematologic system development	• T cell activation (2.2E-12)	• SP_PIR_KEYWORDS
	• Immune system development	• T cell mediated immunity (7.4E-14)	• T cell
	• Lymphatic system development		• 1.4E-16
		• Immunoglobulin receptor family member (1.9E-10)
Network 2 HLA⁶	• RNA posttranscriptional modification	• T cell activation (4.0E-7)	• KEGG_PATHWAY
	• Cell-cell signaling	• MHCII-mediated immunity (6.9E-19)	• Antigen processing and presentation
	• Immune response		• 2.2E-12
		• MHC antigen (2.0E-15)
Network 3 TCF3	• Hematologic system development	• Wnt signaling pathway (1.2E-2)	• GOTERM_BP_ALL
	• Immune system development	• mRNA transcription regulation (3.2E-8)	• Regulation of cellular process
	• Lymphatic system development		• 3.9E-9
		• Other transcription factor (1.1E-5)

¹See Table 3A. BCL2, B-cell CLL/lymphoma 2; HLA, major histocompatibility complex; TCF3, transcription factor 3.
^2,3,4See Table 3A.
⁵There is also a downregulated network on day three (see Table 4B) having BCL2 as the central node. Six of the 35 focus genes (17 percent) are different between the day one and the day three BCL2-related networks.
⁶This network contains multiple HLA genes (HLA-DMA, HLA-DMB, HLA-DOA, HLA-DOB, HLA-DQA1, HLA-DQB1, HLA-DRA, and HLA-DRB1). There is also a downregulated network on day three that contains all of these HLA genes except for HLA-DMA and HLA-DRA. Overall, 13 of the 35 focus genes (37 percent) are different between the day one and the day three HLA-related networks.

ISSN: 1528-3658