Figure 3

Western blot analysis of IGFI/IGF-IR pathway activation in leiomyoma and myometrial tissues.

(A,B) Phosphorylated IGF-IRβ (P < 0.04), Shc (P < 0.05), and MAPKp44/42 (P < 0.02) expression levels were significantly higher in leiomyomas (L) compared with myometrial (M) tissue samples. There was some expression of phosphorylated and total IRS-I and AKT, but no significant differences were found between tumor and myometrial tissue samples. There was very minimal expression of PI3K. Western blot analysis was done at least three times in three independent leiomyoma and matched myometrial tissue preparations from eight patients with comparable results. The band images shown represent four of eight patient samples. The phospho/total ratio data was expressed as mean ± SE in eight patient samples.