Figure 5

Proliferative effects of IGF-I on uterine leiomyoma (UtLM) cells.

IGF-I (100 ng/mL) increased UtLM cell growth in a time-dependent manner. The UtLM cells had significantly higher growth rates when cultured in media with FBS (P < 0.0001) or with charcoal/dextran stripped (SS) media plus IGF-I (P < 0.0001) compared with UtLM cells cultured in media without FBS (SF), or with charcoal/dextran stripped media without IGF-I, or with IGF-I in the absence of SS. Note that UtLM cell growth in SS plus IGF-I medium equaled UtLM cell growth in full FBS medium at 9 days after treatment. The proliferative effect of IGF-I was inhibited by a neutralizing antibody to IGF-IRβ (P < 0.0002). The MTT cell proliferation assay was repeated at least three times with comparable results. The absorbance of MTT was expressed as means ± SE of 8 or 16 wells in 96 wells plates for each treatment condition.