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Figure 1 | Molecular Medicine

Figure 1

From: Upregulated ATM Gene Expression and Activated DNA Crosslink-Induced Damage Response Checkpoint in Fanconi Anemia: Implications for Carcinogenesis

Figure 1

Heightened activation of ATM-p53 axis in FANCA-mutant cells compared with gene-complemented cells. In FANCA-mutant primary fibroblast cells, basal expression of ATM and p53 and phosphorylation of ATM (Ser1981) and p53 (Ser15) induced by IR or MMC were upregulated, compared with identically treated isogenic mutant cells transduced with wild-type FANCA. (A) GM1309C and isogenic control cells transduced with wild-type FANCA; (B) GM16631 and isogenic control cells transduced with wild-type FANCA. Left columns: cells were harvested and lysed 2 hrs after γ irradiation at indicated doses. Right columns: Cells were harvested and lysed 24 h after MMC treatment at indicated doses. (C) HSC72 and isogenic control cells transduced with wild-type FANCA. Postirradiation samples are shown.

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Molecular Medicine

ISSN: 1528-3658