Figure 1

siRNA effects on the protein and mRNA levels of the respective targets. (A) Two siRNAs for each target gene were delivered to CSMCs from donor 1 by Cellfectin and the protein levels evaluated 3 d after transfection; representative blots are reported. (B) The most effective siRNA among each couple was delivered by Metafectene and the protein levels evaluated (CSMCs from donor 1), shown are representative blots. (C) Cumulative data obtained from both Cellfectin and Metafectene transfection are indicated; data, reported as mean ± SEM, n = 5, were normalized to GAPDH and expressed as ratio of siGL2 treatment, #P < 0.02 compared with control. (D) Target mRNA levels were evaluated after siRNA delivery either by Cellfectin (CSMCs from donors 1 and 2) or (E) Metafectene (CSMCs from donor 2); 28S transcript levels were used to normalize the data; NTC, non-treated cells; siGL2, control siRNA-treated cells, data are expressed as mean ± SEM, n = 4. *P < 0.001 and ⁺ P < 0.05 compared with control.