Figure 3

Time course effects of the selected siRNAs on CSMC proliferation. (A) Inhibition of CSMC proliferation, as evaluated by BrdU incorporation, lasted up to d 5 post transfection; the kinetics of cell proliferation reduction for siRNA-treated cells was mathematically evaluated by equation 1 (black lines); cumulative data from both donors are expressed as mean ± SEM, n = 6. (B–D) Proliferation inhibition was paralleled by a concomitant reduction of target proteins; shown are representative blots from donor 1 performed using Cellfectin as transfectant; #all the numbers reported below the blots derive from band quantification normalized to GAPDH and are expressed as the ratio of siGL2 treatment; *P < 0.02 compared with controls, n = 4; NTC, nontreated cells; siGL2, control siRNA-treated cells.