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Figure 4 | Molecular Medicine

Figure 4

From: Role of E2F1-Cyclin E1-Cyclin E2 Circuit in Human Coronary Smooth Muscle Cell Proliferation and Therapeutic Potential of Its Downregulation by siRNAs

Figure 4

siRNA effects on CSMC proliferation in conditions of hyperglycemia or in asynchronous cells and on CSMC migration. (A) Three d after transfection, selected siRNAs were able to reduce the proliferation of CSMCs cultivated in conditions of high glucose (22 mmol/L) compared with controls (*P < 0.05). (B) Selected siRNAs were able to reduce the proliferation of asynchronous CSMCs (#P < 0.05, comparing siRNA-treated to control asynchronous cells); NTC, nontreated cells; siGL2, control siRNA-treated cells; cumulative data from both donors are expressed as mean ± SEM, n = 4. (C) Three d after transfection, the migration rates of siRNA-treated cells were tested as detailed in Materials and Methods; the selected siRNAs were able to reduce the migration of synchronous CSMCs cultivated in conditions of normal glucose compared with controls (§P < 0.04); cumulative data from both donors are expressed as mean ± SEM, n = 15.

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Molecular Medicine

ISSN: 1528-3658