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Figure 5 | Molecular Medicine

Figure 5

From: Role of E2F1-Cyclin E1-Cyclin E2 Circuit in Human Coronary Smooth Muscle Cell Proliferation and Therapeutic Potential of Its Downregulation by siRNAs

Figure 5

Interrelation among E2F1, cyclin E1 and E2 in CSMCs. Three d after transfection, each of the target genes was depleted by the respective siRNAs and the protein levels (A) of the other two target genes evaluated: in the upper panel, representative blots from three independent experiments are reported (CSMCs from donor 2), in the lower panel the total data are shown (CSMCs from donors 1 and 2); #all the numbers reported below the blots are derived from band quantification normalized to GAPDH and are expressed as a ratio of siGL2 treatment; §protein levels were reduced at d 5 post transfection; in (B) the mRNA levels corresponding to the data shown in (A) are reported (28S transcript levels served as normalizator); cumulative data from both donors are expressed as mean ± SEM, n = 6; *P < 0.05 compared with control.

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