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Figure 2 | Molecular Medicine

Figure 2

From: The Synergistic Effect of Tautomycetin on Cyclosporine A-Mediated Immunosuppression in a Rodent Islet Allograft Model

Figure 2

In vitro cytotoxicity test of TMC. (A) Toxicity of TMC was determined by counting the number of viable islets at different time intervals after treatment. Isolated fresh rat islets were cultured at 37°C with or without TMC (0.1, 0.26, 0.6 and 1 µg/mL) or CsA (2),6 and 10 µg/mL) for 3 d. On d 1 and 3 of culture, islets were counted to determine total recovery (gray and black bars, respectively). The ratio of islet number recovered after incubation relative to the initial number (empty bar) was taken as the measure of islet viability. (B,C) Rat splenocytes were cultured under different concentrations of TMC or CsA for 3 d (respectively). At the end of culture, viable splenocytes were detected by a WST-1 assay. O.D., optical density; conc., concentration.

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