Figure 6
Western blot analysis of phospho-MYPT-1, ROCK1 and phospho-ERK1/2 in rat hearts of different groups (I/R, IPC, IPC + PD98059, IPC + fasudil, IPC + PD98059 + fasudil, IPC + DMSO). The amount of phospho-ERK1/2 increased in the IPC group (*P < 0.05 versus I/R), whereas the amount of phospho-MYPT-1 and ROCK1 attenuated in the IPC group (*P < 0.05 versus I/R). After administration of PD98059, the amount of phospho-MYPT-1 and ROCK1 significantly increased (#P < 0.05 versus the IPC group). The molecular weight for phospho-MYPT1 (Millipore) was 80 kDa.