Figure 2

Venn diagram. For each sample, RNA was extracted and after being converted to biotin-labeled cRNA was hybridized to an Affymetrix HG-U133Av2 GeneChip. After image analysis, probe-set expression summaries were calculated by using the robust multiarray average. Thereafter, probe-set-level linear models were fit such that probe-set expression was modeled by HCV status, alcohol status, and the interaction between HCV and alcohol. Of particular interest were those probe sets for which all three terms (HCV, Alcohol, and HCV*Alcohol) were significant. To control for multiple hypothesis tests, probe sets were significant at an α level of 0.0001.