Figure 3

BChE-K prevelance in stroke patients. (A) Cell and tissue sources expressing the BChE gene, located on the reverse genomic strand of chromosome 3 and corresponding HG-U133_PLUS_2 probe sets. ID: BChE — 205433_AT probe set numbers are noted from right to left and known genomic annotations (found by ensemble (http://www.ensemblestudios.com)) are marked. Bar graph and inset: Tissues and cell types with highest expression levels of the human BChE gene. Shown is total signal intensity of BChE mRNA measured by Affymetrix exon arrays (human s_t v1) sample data sets in selected tissues (raw data downloaded from www.affymetrix.com and normalized using the Affymetrix expression console tool with the PLIER algorithm). Inset: whole blood and lymph node data adapted from GeneCards’ GNF SymAtlas (Human GeneAtlas GNF1H, v1.2., Build 20080303-1059, developed by the Bioinformatics Team at GNF (http://symatlas.gnf.org) and normalized by gcRMA). Note variable intensity of labeling for different probe sets and the prominent logarithmically normalized labeling values in nucleated blood cells. The BChE gene yields only one transcript. (B) BChE gene structure, exonic composition and the 3′-terminal DNA and C-terminal amino acid sequences of BChE-U and -K. The amino acid substitution (framed) is shown above the DNA sequence. (C) Histograms of measured BChE activity in stroke patient carriers of the UU, UK and KK genotypes and matched controls. Units are nmole acetylthiocholine hydrolized/min/mL serum.