Table 1 Primers used for amplification.
From: Stearoyl-CoA Desaturase-1 Is Associated with Insulin Resistance in Morbidly Obese Subjects
Gene | NCBI database sequences | Sense primers | Antisense primers |
|---|---|---|---|
SCD1 | NM_005063 | 5′-CAGTGTGTTCGTTGCCACTT-3′ | 5′-GGTAGTTGTGGAAGCCCTCA-3′ |
ATPase p97 | NM-007126.3 | 5′GACTCTGCTGAAGGGTCTGG3′ | 5′ATCCGTGAATCCATCGAGAG3′ |
SREBP-1 | NM-001005291 | 5′-TGCATTTTCTGACACGCTTC-3′ | 5′-CCAAGCTGTACAGGCTCTCC-3′ |
β-Actin | NM_001101 | 5′-TACAGCTTCACCACCACGGC-3′ | 5′-AAGGAAGGCTGGAAGAGTGC-3′ |