Skip to main content

Table 2 Affinity of HNF-1α wild-type and mutant proteins expressed in Cos7 for 1A and 4AP2 probes.a

From: Differential Effects of HNF-1α Mutations Associated with Familial Young-Onset Diabetes on Target Gene Regulation

mHNF-1α mutation

Ka (nmol/L) 1A probe (n)

Ka (nmol/L) 4AP2 probe (n)

None

0.32 ± 0.042 (12)

0.4 ± 0.04 (4)

V133M

1.4 ± 0.26b (4)

0.67 ± 0.05b (3)

T196A

0.27 ± 0.04 (4)

ND

E235G

0.73 ± 0.09b (4)

0.45 ± 0.04 (3)

R271W

1.33 ± 0.16c (4)

1.0 ± 0.07b (4)

P378R

0.26 ± 0.036 (3)

ND

  1. aRelative amounts of protein were determined using Western blot. Results are shown as mean ± SEM of n independent experiments. For each experiment, equal amounts of wild-type and mutant proteins were used. EMSA was performed using constant amounts of proteins and increasing amounts of 32P-labeled probes (0.042, 0.084, 0.168, 0.336, 0.420, 0.672, 1.34, 2.68, 5.36 and 10.2 nmol/L). Kawas calculated as described in “Materials and Methods.” ND, not determined.
  2. bP < 0.05.
  3. cP < 0.01.
Back to article page

Molecular Medicine

ISSN: 1528-3658