Figure 3

ALDH2 dehydrogenase activity analysis. (A) The effect of NAC and α-LA treatment on mitochondrial aldehyde dehydrogenase 2 (ALDH2) activity in isolated rat hearts from control (Con), untreated diabetic (Dia), diabetic treated with NAC (NAC) and diabetic treated with α-LA (α-LA) rats. ALDH2 activity was determined by measuring the conversion of propionaldehyde to its propionic acid product. ^*P < 0.05 versus control group; ^**P < 0.05 versus diabetic group. Data are mean ± SEM (n=6-8). (B) The effect of dithiothreitol (DTT) on ALDH2 activity in isolated heart mitochondria from control or diabetic rats. ^*P < 0.05 versus control group; ^**P < 0.05 versus diabetic group. Data are mean ± SEM (n=4). (C) Representative Western blot showing various cellular markers in total homogenate (Homo) and isolated mitochondria (Mito) prepared from rat hearts.