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Figure 2 | Molecular Medicine

Figure 2

From: Liver Regeneration Associated Protein (ALR) Exhibits Antimetastatic Potential in Hepatocellular Carcinoma

Figure 2

Expression of ALR in HCC cells affects markers of EMT. (A) Immunofluorescence analysis revealed strongly enhanced ALR expression in HepG2-ALR (i) compared with HepG2 (v) cells. HepG2-ALR cells demonstrated reexpression of EMT markers E-cadherin (ii) and ZO-1 (iii), whereas HepG2 cells showed no immunosignal (vi, vii). PHH were used a positive control for E-cadherin (iv) and ZO-1 (viii) immunostaining. (i, v-vii, magnification 400x; ii-iv, viii, magnification 650x). (B) Western blot analysis confirmed expression of E-cadherin and ZO-1 in HepG2-ALR compared with HepG2 cells. Furthermore, expression of SNAIL was not detected in HepG2-ALR, but was found in HepG2 cells. (C) RT-PCR and protein activity analysis of matrix metalloproteinases in HepG2-ALR compared with HepG2 cells revealed reduction of MMP1 and MMP3 mRNA expression and activity, respectively (three independent experiments, mean ± SD, *P < 0.05).

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