Figure 9
From: Differential Roles of Estrogen Receptors α and β in Control of B-Cell Maturation and Selection
BCR signaling is altered by E2 and PPT. (A,B) BCR signaling in transitional B cells of BALB/c mice treated with E2, PPT or vehicle. B cells were incubated with or without 20 µg/mL anti-IgM F(ab)′2, and Erk phosphorylation was determined by flow cytometry. B cells from E2 and PPT-treated B cells displayed a lesser increase in anti-IgM-induced Erk phosphorylation compared with B cells from vehicle-treated mice. (C) An increase in mean fluorescence intensity (MFI) of CD22 expression is evident in WT transitional B cells after E2 and PPT exposure compared with vehicle exposure.