Figure 1

Appearance of MDSCs obtained by cytospin from different clinical conditions. (A) Murine control; (B) murine cancer; (C) human cancer; (D) murine sepsis; (E) murine trauma; (F) murine autoimmunity. GR-1⁺ splenocytes were obtained from a variety of experimental conditions, including healthy controls (Left, unpublished data; Right (48)), animals with transplantable tumors (Left and Right, unpublished data), human cancer (18), after trauma (48), sepsis (Left (12) and Right (28)) and experimental autoimmune disease (56). Although the photomicrographs were obtained under different experimental conditions, the populations of GR-1⁺ cells differ dramatically not only within the same condition, but also between conditions. MDSCs phenotypically vary from more mature-appearing neutrophils, to ringed cells, to more immature blastlike-appearing cells. Arrows indicate different subsets of MDSCs: granulocytic (black), ringlike (red), monocytic (orange) and blast-like (green).