Figure 4

Expansion of Gr-1⁺CD11b⁺ cells in spleen and lymph nodes of burned and infected mice. (A), Flow cytometric scattergram showing populations of GR-1⁺CD11b⁺ cells in a spleen from a control mouse. Details concerning the experimental methods are contained in the Supplementary Methods. (B), Flow cytometric scattergram showing expansion of GR-1⁺CD11b⁺ cells in a spleen from a mouse that underwent a 10% burn and Pseudomonas infection harvested on d 7. (C), Summary data of the effect of the burn and Pseudomonas infection on splenic GR-1⁺CD11b⁺ cells. (D), Summary data of the effect of the burn and Pseudomonas infection on lymph node GR-1⁺CD11b⁺ cells. In both lymphoid organs, there was a significant increase in the expansion of GR-1⁺CD11b⁺ cells from the Pseudomonas infection alone experimental group as well as the 10% burn + infection group as compared with controls. Values represent the median and estimated quartiles of five animals per group, using a box plot representation, since the values failed tests of normality and equal variance. * indicates that the control group differed only from the groups of mice receiving the Pseudomonas infection, either alone or superimposed on the burn; P < 0.05.