Skip to main content
Figure 1 | Molecular Medicine

Figure 1

From: Selective Antihypertensive Dihydropyridines Lower Aβ Accumulation by Targeting both the Production and the Clearance of Aβ across the Blood-Brain Barrier

Figure 1

Dose-dependent effect of antihypertensive calcium channel blockers on Aβ production by 7W CHO cells overexpressing APP. (A) Effect of a 24 h treatment with different calcium channel blockers on Aβ1−40 production. Aβ1−40 values were expressed as a percentage of the Aβ1−40 values obtained in the vehicle treatment conditions ± S.E.M. ANOVA reveals statistically significant main effect of amlodipine (P < 0.001), nilvadipine (P < 0.001), nitrendipine (P < 0.05) and nimodipine (P < 0.002), but no statistically significant main effect of felodipine (P= 0.1), isradipine (P= 0.055), nifedipine (P= 0.245), diltiazem (P= 0.09) or verapamil (P = 0.173) on Aβ1−40 production. Post hoc analyses show significant differences between Aβ1−40 values in untreated cells and cells treated with amlodipine at 1 (P < 0.02), 2.5 (P < 0.001), 5 (P < 0.001) and 10 µmol/L (P < 0.001), with nilvadipine at 5 and 10 µmol/L (P < 0.001), with nitrendipine at 5 and 10 µmol/L (P < 0.001) and with nimodipine at 5 and 10 µmol/L (P < 0.001). (B) Effect of a 24 h treatment with different calcium channel blockers on Aβ1−42 production. Aβ1−42 values were expressed as a percentage of the Aβ1−42 values obtained in the vehicle treatment conditions ± S.E.M. ANOVA reveals statistically significant main effect of amlodipine (P < 0.001), nilvadipine (P < 0.001), isradipine (P < 0.001) and nimodipine (P < 0.001), but no statistically significant main effect of felodipine (P= 0.577), nitrendipine (P = 0.293), nifedipine (P= 0.663), verapamil (P= 0.968) and diltiazem (P= 0.308) on Aβ1−42 production. Post hoc analyses show significant differences between Aβ1−42 values in untreated cells and cells treated with amlodipine at 1, 2.5, 5 and 10 µmol/L (P < 0.005), with nilvadipine at 2.5, 5 and 10 µmol/L (P < 0.01), with nimodipine at 2.5, 5 and 10 µmol/L (P < 0.002) and with isradipine at 2.5, 5 and 10 µmol/L (P < 0.001). (Average Aβ1−40 values observed in the control conditions were 2,092.3 pg/mL whereas Aβ1−42 values were 189.5 pg/mL).

Back to article page