Figure 5
From: Acute Stress Elicited by Bungee Jumping Suppresses Human Innate Immunity
Kinome profiling of human peripheral blood reveals acute stress-induced activation of noncanonical glucocorticoid receptor signal transduction. Peptides displaying differential significant phosphorylation by leukocyte lysates prepared from volunteer blood obtained 2 h before and directly after jumping were used to construct provisional signal transduction schemes, detailing the effects of acute stress on signaling biochemistry. Inconsistent peptides (for example, from the protein kinase C (PKC)α-responsive peptides, six peptides showed upregulation before jumping, whereas five other peptides were upregulated after jumping) were discarded from the provisional signal transduction scheme. From the profiles obtained, a clear noncanonical glucocorticoid receptor signaling signature emerged. Arrows represent biochemical connection (bibliome). Green circles represent upregulation after the jump; red circles represent downregulation after the jump. Blue boxes depict kinases that are reported intermediates of noncanonical glucocorticoid signaling.