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Figure 5 | Molecular Medicine

Figure 5

From: Bone Marrow Stromal Cell-Derived Vascular Endothelial Growth Factor (VEGF) Rather Than Chronic Lymphocytic Leukemia (CLL) Cell-Derived VEGF Is Essential for the Apoptotic Resistance of Cultured CLL Cells

Figure 5

Blockage of secreted VEGF protein by addition of a VEGF-neutralizing monoclonal antibody (mAb). (A) Neutralization of secreted VEGF in a stromal cell/CLL cell coculture using the monoclonal antibody mAb293 (R&D Systems) (10 µg/mL) significantly reduced the coculture-mediated survival support for CLL cells (P = 0.030, paired two-tailed t test). Survival advantage was calculated by subtracting survival (%) in monoculture from survival (%) in coculture. (B) Annexin V-FITC/PI status of one representative sample is displayed as a flow cytometry dot blot. The left lower quadrant represents annexin V-FITC/PI-negative cells (alive), and the right upper quadrant represents annexin V-FITC/PI-positive cells (dead). (C) Addition of the VEGF-neutralizing antibody mAb293 (10 µg/mL) to CLL cells in a monoculture setting did not significantly influence CLL cell survival. Annexin V-FITC/PI status was analyzed on FACSCanto. *P < 0.05.

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Molecular Medicine

ISSN: 1528-3658