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Figure 5 | Molecular Medicine

Figure 5

From: Macrophage Migration Inhibitory Factor Mediates Hypoxia-Induced Pulmonary Hypertension

Figure 5

Increased hypoxia-induced cell proliferation in a human lung fibroblast cell line. Human lung fibroblasts (CCL-210) were cultured in normoxia or 1% oxygen atmosphere (hypoxia). After 24 h, cell proliferation was evaluated by both BrDU incorporation (A) and confirmed by cell enumeration (72 h) of hypoxia (B). Cell proliferation in hypoxia was significantly increased compared with growth in normoxia in each evaluation. The increased growth was associated with increased accumulation of (C) MIF in the culture medium; and (D) intracellular mRNA encoding MIF; (E) ISO-92, but not the vehicle DMSO, inhibited proliferation in a dose dependent manner. Data represents at least 3 independent experiments: *P < 0.05, **P < 0.01 versus normoxia; ##P < 0.01 versus DMSO control group.

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