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Figure 1 | Molecular Medicine

Figure 1

From: Interferon Regulatory Factor-1 Regulates the Autophagic Response in LPS-Stimulated Macrophages through Nitric Oxide

Figure 1

Macrophages from IRF-1 KO mice exhibit decreased apoptosis and increased autophagy during endotoxemia. (A) Following PBS or LPS (20 mg/kg) administration for 16 h, splenic macrophages were isolated from WT and IRF-1 KO mice and analyzed by Western blot for LC3B I/II and cleaved caspase-3. (B) Following PBS or LPS (20 mg/kg) administration for 16 h, alveolar macrophages were isolated from WT and IRF-1 KO mice and analyzed by Western blot for LC3B I/II and cleaved caspase-3. (C) Following PBS or LPS (20 mg/kg) administration for 16 h, liver Kupffer cells were isolated from WT and IRF-1 KO mice and analyzed by Western blot for LC3B I/II and cleaved caspase-3. Results are representative of three separate independent experiments.

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