Figure 5
Similar soluble intracellular Aβ species in the brain of APPSwe/PS1 and APPSwe/PS1/CCR2−/− mice transplanted with WT (GFP) or CCR2−/− BMCs. Hemibrain extracts from soluble intracellular proteins (A, C) of 6-month-old APPSwe/PS1 and APPSwe/PS1/CCR2−/− mice harboring WT GFP or CCR2−/− cells in their bone marrow were assessed by Western blot. Densitometric analysis was used to measure the intensity of each band, which was normalized to β-actin corresponding values. Each Aβ species ratio (Aβ/β-actin) was represented for intracellular proteins (B, D). All groups of mice had similar soluble intracellular Aβ species levels. Results are expressed as the mean ± SEM; n = 4−5. One-way ANOVA was performed using Dunnett or Tamhane post hoc tests. OD, optical density; ■, APPSwe/PS1; 
, GFP → APPSwe/PS1; 
, CCR2−/− → APPSwe/PSl; 
, APPSwe/PS1/CCR2−/−; 
, GFP → APPSwe/PS1/CCR2−/−.