Figure 1From: β-Amyloid Carrying the Dutch Mutation Has Diverse Effects on Calpain-Mediated Toxicity in Hippocampal NeuronsReduced number of synaptic contacts in mature hippocampal neurons treated with E22Q Aβ. (A–F) Twenty-one days after plating, untreated controls (A, B) and neurons incubated with WT (C, D) or Dutch (E22Q) (E, F) Aβ (10 µmol/L) for 24 h were immunolabeled with tubulin (A, C, E) and synaptophysin (B, D, F) antibodies. Note the similar decrease in the numbers of synaptophysin immunoreactive spots in neurons treated with either form of Aβ compared with untreated controls. (G) Quantification of the number of synaptophysin-positive puncta per cell in untreated neurons and in neurons treated as described in A–F. Values represent the mean ± SEM for five independent experiments per condition (n = 90 cells/condition). **Differs from untreated control, P < 0.01. Scale bar: 50 µm.Back to article page