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Figure 3 | Molecular Medicine

Figure 3

From: RETRACTED ARTICLE: Redox Modification of Cysteine Residues Regulates the Cytokine Activity of High Mobility Group Box-1 (HMGB1)

Figure 3

Redox dependent effect on HMGB1-induced NF-κB activation in macrophages. Western analysis of the nuclear localization of the NF-κB p65 subunit following the stimulation of cultured macrophages for 1 h with 5 µg/mL of HMGB1 or 4 ng/mL of LPS. HMGB1 was exposed to either H2O2 (50 mmol/L, 120 min) or DTT (5 mmol/L, 120 min) prior to the assay. For HMGB1 preparations that had included DTT, HMGB1 was then oxidized with 50 µmol/L H2O2 β actin expression was also measured and used as a loading control. All HMGB1 preparations were characterized by LC-MS/MS prior to cell treatment with respect to cysteine redox status as described in Figure 1. Data shown are representative of three independent experiments. A, control (PBS); B, HMGB1 (molecule B + H2O2); C, HMGB1 (molecule C + DTT); D, LPS; E, HMGB1 (molecule A); F, HMGB1 (molecule C + H2O2).

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