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Figure 3 | Molecular Medicine

Figure 3

From: MicroRNA-218 Inhibits Cell Cycle Progression and Promotes Apoptosis in Colon Cancer by Downregulating BMI1 Polycomb Ring Finger Oncogene

Figure 3

Transfection of miR-218 decreases BMI-1. (A) Overexpression of miR-218 was demonstrated in HCT116 and HT29 cell lines by quantitative RT-PCR. (B) Ectopic expression of miR-218 downregulated the BMI-1 mRNA level in HCT116 and HT29 cells, as determined by quantitative real-time PCR. (C) miR-218 decreased the BMI-1 protein level in HCT116 and HT29 cells, as determined by Western blot. The BMI-1 protein band intensities were quantified and normalized to GAPDH intensities (n = 3, mean ± SD). (D) Levels of BMI-1 mRNA is upregulated in primary colorectal tumors compared with their adjacent normal tissues (n = 46). (E) BMI-1 mRNA is upregulated in colon cancer cell lines compared with five normal colon tissues.

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Molecular Medicine

ISSN: 1528-3658