Figure 3

SPRR3 increases cell proliferation. The cell proliferation rates of control SW480 cells and SW480 cells stably expressing SPRR3 (SW480/SPRR3) were measured by a CCK8 cell proliferation assay. The results are presented in terms of the daily proliferation rate (A). The SW480 and SW480/SPRR3 cells were harvested and stained with propidium iodide and then they were subjected to a flow cytometer for cell cycle analysis. The bars represent the cell population at each stage of the cell cycle (B). Anchorage-independent cell growth was measured by a fluorescence plate reader after SW480 and SW480/SPRR3 (1 ×10⁴) cells were seeded and cultured for 14 d. The colonies were visualized by crystal violet staining (C). RFU, relative fluoresce unit. The data are presented as the mean ± SEM.